منابع مشابه
Ethidium bromide-resistant mutant of Bacillus subtilis.
An ethidium bromide-resistant mutant (EB8) derived from a Marburg strain of Bacillus subtilis was found to be conditionally resistant to 10 mug of ethidium bromide per ml. Expression of resistance is complete only during vegetative growth at incubation temperatures above 30 C in complex medium or minimal medium supplemented with Casamino Acids. Strain EB8 is cross-resistant to acriflavine and p...
متن کاملA deoxyribonucleic acid polymerase I-deficient mutant of Bacillus subtilis.
A methyl methanesulfonate sensitive mutant of Bacillus subtilis appears to lack the enzyme DNA polymerase I. The absence of polymerase I permits the detection of a second DNA polymerizing enzyme in the mutant. This second polymerase is inhibitable by sulfhydryl antagonists, and shows maximal activity on double-stranded DNA substrates with many single strand breaks. Poly[d(A-T)] is less efficien...
متن کاملPhosphohydrolases of a Bacillus subtilis mutant accumulating inosine and hypoxanthine.
Although adenine-requiring auxotrophs of Bacillus subtilis accumulate large quantities of inosine or hypoxanthine, or of both, they do not accumulate inosine-5'-monophosphate (IMP). Experiments directed at understanding this phenomenon were conducted with an adenineless auxotroph and with a mutant derived from it which lacked alkaline phosphohydrolase. It was found that B. subtilis contains fou...
متن کاملMutant of Bacillus subtilis lacking exo-beta-N-acetylglucosaminidase activity.
A procedure for the isolation of exo-beta-N-acetylglucosaminidase mutants, by using a plate assay method incorporating a fluorescent substrate, has been developed. A mutant lacking exo-beta-N-acetylglucosaminidase activity has been isolated and shown to grow, divide, autolyze, and sporulate as well as the parental strain.
متن کاملTranscriptional Profile of Bacillus subtilis sigF-Mutant during Vegetative Growth
Sigma factor F is the first forespore specific transcription factor in Bacillus subtilis and controls genes required for the early stages of prespore development. The role of sigF is well studied under conditions that induce sporulation. Here, the impact of sigF disruption on the transcriptome of exponentially growing cultures is studied by micro-array analysis. Under these conditions that typi...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1982
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.149.2.635-641.1982